Archive: Medical

  • Clinical Significance of Telomerase Activity and Fas Expression in Egyptian Children with Acute Leukemia

    Telomerase is a ribonucleoprotein enzyme that maintains protective structures at the ends of eukaryotic chromosomes. Telomeric repeat amplification protocol (TRAP) was used to determine telomerase activity (TA) in WBCs of 45 pediatric patients with acute lymphoblastic leukemia ( ALL ) before treatment and after complete remission and in WBCs of 6 healthy donars that were selected as a control group. CD95 (Fas / APO-1) is a cell surface receptor able to trigger apoptosis in a variety of cell types. The expression of CD95 antigen on leukemia blasts from the 45 patients before treatment and on aleukocytes after complete remission and on leukocytes of the control group were determined by flow cytometry. The results obtained in the present study indicate that, telomerase activity and Fas expression were significantly increased in the studied cases before treatment compared to the control group. After treatment, there was significant decrease in TA while there was significant increase in Fas expression compared to before treatment. These results suggest that telomerase activity and Fas expression might be useful in diagnosis and follow-up of ALL in pediatric patients.

    Download PDF 'Sylvia A. Boshra, Zeinab A. Hassan, Atef T. Fahim, Ibrahim M. Abdel Salam and Mohammed M. Elmazard'