In Press: Medical

  • Biopharmacodynamical Effect of Clindamycin and Spectinomycin Combination in Experimentally E.Coli Infected Broilers

    Antimicrobials are co-administered frequently worldwide in medical as well as veterinary practices. Often, the therapeutic efficiency of two antimicrobial drugs may induce adverse effects. In this study, the possibility of a clindamycin/spectinomycin interaction effect and the efficacy of the combination were investigated in 23 day-old chickens experimentally infected with E. coli. The challenged chickens were orally treated with Clindamycin (11mg/ kg body weight) or spectinomycin (51.1 mg/L) for 5 successive days with a combination of both drugs at therapeutic and half-therapeutic doses. Two blood samples were collected from five chickens of each group on the 4th day during the treatment course and on the 2nd, 7th, 14th and 21st days post-treatment. The results revealed that co-administration of clindamycin/spectinomycin reduced the severity of clinical signs and altered the biochemical function parameters related to liver, kidney and of oxidative stress. The effect of both drugs was short-lived, and most of the parameters returned to normal within 2 weeks after administration of them. It is concluded that, the therapeutic doses co- administration of clindamycin/spectinomycin reduced the severity of E. coli infection clinical signs and showed significant alterations in some liver and kidney function parameters as well as oxidative stress. This effect is short-lived and most of the parameters went back to normal within 2 weeks post drug(s) administration.

    'Kadry Mohamed Sadek and Hazem Mohammed Shaheen '
  • Immunosuppressive Toxic Effects of Ochratoxin A in Mature Male Albino Rats

    Ochratoxin A (OTA) is a mycotoxin produced by species of the genus Aspergillus and panicillium. Human exposure has been demonstrated worldwide and its origin seems to be the intake of contaminated foods. Immunotoxic and genotoxic effects of OTA were investigated in mature male albino rats, when treated with garage in a close of 150, 300 and 450 OTA μg/kg B.wt. for 90 days. In the context several immune function assays were performed and bone marrow smears were obtained and stained in order to analyse micronuclei in polychromatic erythrocytes. The response of splenocytes to sheep red blood cells was decreased in a dose –dependent manner. However, slightly statistically significant differences were obtained. Cyhtotoxic T lymphocytes activity was lower in the animals exposed to 150 OTA μg/kg B.wt. but was not modified in the groups exposed to 300 & 450 μg/kg B.wt.. The number of micronuclei in bone marrow polychromatic erythrocytes was slightly increased with respect to the control at any dose.

    'Magdy F. Abou El Fotoh, Kamel M.A. , Nora E.A. and Magda Hassan'
  • Biochemical Alteration in Brain Natriuretic Peptide and Electrolytes in Myocardial Patients

    Brain Natriuretic Peptide (BNP) and electrolytes are important biomarker in myocardial infraction. To evaluate the relationship between Electrolyte (Sodium, Potassium, Chloride), BNP, Nitric Oxide, and Immunoglobulin in patients with myocardial infarction (MI), 25 patients with heart failure and 10 clinically healthy subjects (control), were used. The results of the present study showed the association between BNP and electrolytes, nitric oxide in addition to immunoglobulin. The results showed a decrease in sodium, potassium, chloride, and immunoglobulin except IgE and increase in BNP and NO. These parameters may be regarded as predictors or risk factors of MI.

    'Abd El-Maksoud H, Mohamed Kh and M Eman '
  • Evaluation of Chitosan Membrane with or without Autologous Platelet Rich Plasma

    Chitosan is known to be natural polymers and it is available, biodegradable, biocompatible, and non-toxic and has antimicrobial and anti-inflammatory effects. Platelet rich plasma (PRP) is an autologous source of platelet derived growth factor and transforming growth factor beta that play a role in periodontal regeneration. The present study was designed to evaluate and compare chitosan membrane with or without PRP versus CTG in the treatment of Miller Class I buccal gingival recession ≥ 4 mm in width. Thirty sites in patients with Miller Class I buccal gingival recession ≥ 4 mm in width were included in this study. The patients were classified randomly into three treatment groups, ten sites for each group. Selected recession sites were treated with one of the following three modalities. Group I: connective tissue graft in addition coronally advanced flap. Group II: chitosan membrane in addition coronally advanced flap. Group III: chitosan membrane, autologous platelet-rich plasma in addition coronally advanced flap. Plaque index (PI), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL), recession height (RH), recession width (RW), digital measurements of area of recession and height of the keratinized tissue (HKT) were recorded at baseline and at 1, 3, 6 and 12 months post surgically, except for PPD & CAL which were assessed at 6 and 12 months post surgically. Gingival thickness (GT) was recorded at baseline and after 12 months post surgically. Percentage of root coverage (RC) was recorded at 1, 3, 6, 12 months and wound healing index (WHI) was recorded at 2 weeks, 1, 3, and 6 months after surgery. All groups showed comparable results at the end of the study period. The three treatment modalities showed a statistically significant reduction in CAL at 6 and 12 months as compared to the mean baseline value. Intergroup comparison showed that group III had significantly less RH, RW, and digital measurement of recession area as compared to group I at one month, while there was statistically insignificant difference between group II and I. On the other hand, the results at 3, 6 and 12 months post-surgery were comparable and statistically insignificant. Difference in all treated groups there was a statistically significant increase in the mean HKT up to 12 months as compared to the mean baseline value. However, CTG lead to more HKT as compared to groups II & III. There was a statistically significant increase in the mean GT after 12 months post-surgery in all treated groups. The mean GT was more significantly increased in group I& III as compared to group II. A statistically significant reduction in WHI in chitosan membrane and chitosan membrane and PRP groups when compared to the CTG group at 2 weeks post-operatively. Clinical results of the present study indicated that, the addition of chitosan membrane with or without PRP to CAF resulted in root coverage comparable to CTG but without the discomfort as in the second surgical procedure and the potential clinical difficulties associated with the donor site surgery. Clinically the addition of PRP to chitosan resulted in an increase gingival thickness as compared to chitosan membrane alone. Gingival thickness in group III (chitosan with PRP) was comparable to CTG.

    'Enas A. Elgendy, Samia A. Darwish, SamiaN. Serag, Amal M. Aldeeb'